CURRICULUM VITAE
1.- PERSONAL INFORMATION
Name: Gonzalo Rafael.
Lastname: Ferreira de Mattos.
Date
of Birth:
Home
address: 6632 Alamo Ave. Apt.1W.
Clayton. MO. 63105.
Work address:
Home Phone Number: (314)-725-5223
Work Phone Number: (314)-362-3677
Fax Number: (314)-362-3446.
E-mail: ferreira@pcg.wustl.edu, ferreira@fmed.edu.uy
2.- ACADEMIC DEGREES
a.-Philosophical
Doctor in Biophysics. (Ph.D.) - PEDECIBA (Program for the Development of
Basic Sciences), University of the Republic, United Nations,
b.-Medical
Doctor (M.D.) - Faculty of Medicine, University of the Republic,
Date of Graduation:
c.-Master
in Biological Sciences (Biophysics) (M.Sc.) - PEDECIBA (Program for the
Development of Basic Sciences), University of the Republic, United Nations,
Date of Graduation:
3.- ACADEMIC SERVICE
a.-
Assistant Professor (III). Biophysics Dept. Faculty of Medicine.
University of the Republic.
b.- Honorary Assistant Professor. Dept. of
Biophysics. CECS.
c.- Instructor. Dept. Of Physiology and Molecular Biophysics.
Dr. Rios’s lab.
d.-
Research Assistant. Dept. Of Physiology and Molecular Biophysics. Dr.
Rios’s lab.
e.- Instructor (II). Biophysics
Dept. Faculty of Medicine. University of the Republic.
f.-
Research Fellow (I). Biophysics Dept. Faculty of Medicine. University of
the Republic.
4.- RESEARCH ACTIVITIES
A.- Publications
a.- Original Articles
i) Corresponding Author
-Two components of voltage-dependent inactivation in Cav 1.2 channels revealed by its gating currents. G.Ferreira, E.Ríos y N.Reyes. (2003). Biophys J. 84(6):3662-78.
ii)
First Author / Intense involvement in all the stages
-Opposite modulation of two High Conductance
Potassium Channels by Neurotransmitters. Santi, C; Ferreira,
G;
-Inactivation of gating currents of L-type calcium channels. Specific role of the a2d subunit. Shirokov, R; Ferreira, G; Yi, J and Rios, E. (1998). J. Gen. Physiol. 111: 807-823.
-Ion-dependent inactivation of barium current through L-type calcium channels. Ferreira, G; Yi, J; Rios, E; and Shirokov, R. (1997). J Gen Physiol. 109: 449‑461.
-Butanedione Monoxime promotes voltage-dependent inactivation of L-type calcium channels in heart. Effects on gating currents. Ferreira, G; Artigas, P; Pizarro, G and Brum, G. (1997). J Mol Cell Cardiol. 29(2): 777‑787.
iii) Collaborations
-Effects of the enantiomers of BayK 8644 on the charge movement of L-type Ca channels in guinea-pig ventricular myocytes. (2003). Artigas P, Ferreira G, Reyes N, Brum G, Pizarro G. J Membr Biol. 193(3):215-27
b.- Chapters in Books
-
Potassium and
Calcium Channels in C. elegans. L. Salkoff, B. Baban, A. Butler, G. Fawcett, G. Ferreira, C.M. Santi, & A. Wei . (2004). Submitted to be
published in C.Elegans II.
- Comparison of the effects of BDM on L-type Ca channels of cardiac and skeletal muscle. Gonzalo Ferreira, Pablo Artigas, Rafael De Armas, Gonzalo Pizarro and Gustavo Brum In Calcium and cellular metabolism: Transport and Regulation. Ed. J.R.Sotelo & J.C.Benech. Published by Plenum Pub Corp. Publication date: June 1997. ISBN: 0306455943.
c.- Reviews
-New Disguises for an Old Channel: Voltage and
Calcium-Activated K+ (MaxiK)
Channels beta subunits. Patricio Orio, Patricio Rojas, Gonzalo Ferreira and Ramón Latorre. News in Physiological Sciences. (2002). 17:156-161.
d.- Thesis
-Inactivacion voltaje-dependiente en canales de Ca2+ L
(Cav1.2) expresados en celulas tsA 201. (Translation – Voltaje-dependent
inactivation in L-type Ca2+ channels (Cav1.2), expressed in tsA 201 cells).
Ph.D. Thesis. (PEDECIBA Library). Faculty of Medicine,
-Registro y caracterización del movimiento
de carga intramembrana en miocitos aislados de miocardio ventricular de cobayo.
(1992) (Translation - Record and characterization of the intramembrane
charge movement in myocytes isolated from the guinea-pig myocardial ventricle).
M.Sc. Thesis. (PEDECIBA Library). Faculty of Medicine,
e.- Abstracts
-Two
novel mammalian high conductance potassium channels are oppositely modulated by
PKC. Ferreira, G; Santi, C;
-The
structural requirements for inhibition by PKC in Slo2.1 channels are located in
the channels’s tail. Santi, C; Ferreira,
G;
-Activation of BK channels by local Ca2+ entry through L-type Ca2+ channels, coexpressed in tsA 201 cells. N.Reyes, L.Salkoff, A.Butler, G.Pizarro, G.Brum and G.Ferreira. XIV International Biophysics Congress (IUPAB), 2002, 103p.
-Efecto de cationes
extracelulares en el componente rápido de inactivaci{on dependiente de voltaje
en Cav1.2 expresado en células tsA 201. G.
Ferreira, N.Reyes, G.Budelli, R. De Armas, G.Brum y E.Ríos. X Congreso
Sociedad Uruguaya de Biociencias (SUB), 2002, B41.
-Effects
of partial deletions of S3 segment in Shaker K+ channel activation kinetics.
C.Gonzalez, G.Ferreira, E.Rosenmann,
F. Bezanilla, O.Alvarez and R.Latorre. Biophys. J. 2002. 82:A234.
-Modulation
by Ca2+ and Mg2+ of mammalian muscle Ca2+ sparks. J.Zhou, A.Gonzalez, G. Ferreira, R.Segura, E.Rios, J.Yi and G.Brum. Biophys.J. 2002.
82:A510.
-Functional
coupling between L-type Calcium channels and BK channels coexpressed in tsA 201
cells. N. Reyes, G. Pizarro, G. Brum, L.Salkoff and G.Ferreira. Biophys.J. 2001. 80:A209.
-Correlation
of surfaces of inactivation of ionic and gating currents in L-type Ca channels
expressed in tsA 201 cell.s. G.Ferreira,
N.Reyes, G.Pizarro, G.Brum and E.Ríos. Biophys.J. 2001. 80:A620.
-Ausencia de inactivaciòn
preferencial de estados cerrados en canales de Ca2+ L. Ferreira, G; Reyes, N; Pizarro, G; Brum, G and Rìos, E. SUB 2000.
Libro de resùmenes.
-Coexpresiòn de canales de Ca2+
L y K+ activados por Ca2+ (BK) en cèlulas tsA 201. Ferreira, G; Reyes, N; Pizarro, G; Brum, G; Salkoff, L and Brandl,
F. SUB 2000. Libro de
resùmenes.
-Efecto de S(-) BayK8644 sobre
las corrientes de compuerta (Ig) del canal de Ca2+ L cardìaco. Artigas, P; Ferreira, G; Reyes, N; Brum, G and
Pizarro, G. SUB 2000. Libro de resùmenes.
-Corrientes de membrana y
transitorios de Ca2+ en cèlulas musculares esquelèticas aisladas de la cola del
renacuajo. Ferreira, G; Reyes, N;
Rìos, E; Pizarro, G and Brum, G. SUB
2000. Libro de resùmenes.
-Fast and slow components of voltage dependent inactivation in L-type Ca2+ channels. Ferreira, G; Yi, J and Ríos, E. Biophys. J. (1999). 76: A340.
- High intracellular calcium affects L-type Calcium Channel gating. G. Ferreira, H.Takeshima, E.Rios and A. Gonzalez. Biophys. J. (1998). 74: A101.
- Effects of the a2d subunit on inactivation of gating currents interpreted with a continuum model of Ca2+ channel gating. R. Shirokov, G. Ferreira and E. Rios. Biophysical Society Meeting (1998).
- Effect of the a2d subunit on voltage-dependent inactivation of cardiac Ca2+ channels. R. Shirokov, G. Ferreira, J. Yi, and E. Rios. Biophys. J. (1997). 70: A183.
- Barium-dependent inactivation of L-type Calcium channels. (1997). G.Ferreira, J.Yi, E.Rios and R. Shirokov. Biophys. J. (1997). 70: A183.
- Inactivation of ionic current and interconversion of gating charge in a1C/b2a channels expressed in a mammalian cell line. Ferreira, G; Rios, E and Shirokov, R. Biophys. J. (1996) 70:A237.
- Similarity of biophysical properties of Ca2+ currents in a1C and a1C/b2a transfected mammalian cells. Shirokov, R, Ferreira, G and Rios, E. Biophys. J. (1996). 70: A183.
-
A comparative study of the effect of Butanedione monoxime (BDM) on L-type ionic
currents in cardiac and skeletal muscle. De Armas, R; Ferreira, G; Gonzalez, S; Brum, G and Pizarro, G. Congress of the
Uruguayan Society for Biosciences.(1995). Libro de resúmenes. Faculty of Medicine,
-
BDM promotes L-type Calcium channel inactivation: Gating current effects. Ferreira, G; Pizarro, G; Brum, G; and
Artigas, P. Satellite Symposium of the XXXVII Congreso Nacional de Ciencias
Fisiologicas. (1994).
- BDM promueve la inactivación del canal
de calcio L: Efectos sobre corrientes de compuerta. XVIII Latinoamerican
Congress of Physiological Sciences. Ferreira,
G; Pizarro, G; Brum, G and Artigas,
P. (1994).
- BDM enhances voltage-dependent inactivation of L-type Calcium channel in heart. Ferreira, G; Maggi, M; Pizarro, G; and Brum, G. Biophys. J. (1993). 64:A202.
-
Effects of Adrenaline on Calcium release in single fibers of frog skeletal
muscle. Brum, G; Gonzalez, S; Ferreira,
G; Maggi, M; and Santi, C. Biophys. J. (1990) 57:A342.
- Efectos de la Adrenalina sobre la
liberación de calcio en musculo esquelético de rana.Brum, G.; Gonzalez, S; Ferreira, G; Maggi, M and Santi, C.
Congreso Iberoamericano de Biofísica. (1989). Libro de resúmenes.
B.- Scientific
Events
a.-
Meetings with presentations
-
40th , 41th , 42th, 43th,
45th , 47th Annual Meeting Biophysical Society.
-
Experimental Biology Meeting. (2001).
- Simposio Satélite de el XXXVII Congreso
Mexicano de Ciencias Fisiológicas. (1994). Cancún, México.
- XVIII Congreso Latinoamericano de Ciencias
Fisiológicas. (1994).
b.-
Invited seminars given at Universities, related to research activities
performed.
- Functional coupling between L-type Ca2+ channels and BK channels expressed in tsA 201 cells. (2001).
Centro de Estudios Cientificos.
Chile.
-Voltage
dependent inactivation in L-type Ca2+ channels. (2001).
-Fast
and slow mechanisms of voltage dependent
inactivation in L-type Ca2+ channels. (1999).
-Ion-dependent
inactivation of Barium currents through L-type Calcium channels. (1996).
-The
effects of BDM on calcium and gating currents of L-type Ca2+ channels in cardiac
myocytes. (1995).
-2,3
Butanedione-Monoxime promotes inactivation of L-type Calcium channel in heart.
Gating current effects. (1994).
c.
– Invited seminars given at International Meetings, related to research
activities performed.
-Inactivation
in L-type Ca channels. April 2001.FASEB meeting (Experimental Biology).
-Voltage
dependent inactivation mechanisms in L-type Ca channels. International Symposia “Ion channels, a glimpse to the
future”. Nov 2000. Invited by CECS,
d. - Events Organized.
-Symposium
“New Perspectives in intracellular Calcium and Calcium sensitive channels”. 2002. Satellite of the
2002 Congress of the International
C.- Professional
Experience
a.- Laboratory Work.
-Molecular
and Cell Biology. Modulation by PKC in Slo-2 channels. (2003-2004). Dr.
Salkoff’s lab.
-Expression
in oocytes, cysteine accesibility, Molecular fluorescence. Ion channel structure predictions. (2001). Dr. Ramon Latorre’s lab. CECS.
-Molecular
Biology training. Development of an artificial ion channel cluster. (2001). Dr.
Salkoff’s lab. Dept. of Anatomy and Neurobiology.
-Expression
of ionic channels in mammalian cells. Cell culture, molecular biology of ion
channels. (1995-99). Dept. Of Physiology and Molec. Biophysics.
-
Expression of Calcium channels in mammalian cells, tissue culture and molecular
biology techniques. (1996-97). Dr. Rios’s lab. Dept. Of Physiology and Molec. Biophysics.
-
Expression of ionic channels and record of currents in oocytes with the
cut-open technique. Training by Dr. Miguel Holmgren in Dr. Rakowski’s lab.
(1994). Dept. of Physiology.
- Studies in cardiac myocytes. Ionic and gating currents of L-type Calcium channels (89-95)
- Studies in skeletal muscle, cut fiber technique (1987-88).
- Development of software for simulation of biochemical reactions. Methods of Euler and Runge-Kutta. (1986-87).
b.-
Experimental setups built by applicant in his lab.
-Ion channel expression and molecular biology. (1999-).
-Patch Clamp. (1990-).
-Isolation of cardiac myocytes (1989-).
c.-
Laboratories with whom I collaborate.
-Dr.
Lawrence Salkoff. Dept. of Anatomy and Neurobiology.
-Dr. Eduardo Rios. Dept. of
Physiology and Molecular Biophysics.
-Dr.
Ramon Latorre. Dept. of Biophysics. CECS.
-Dr.
Gustavo Brum. Dept. of Biophysics.
-Dr. Gonzalo Pizarro. Dept. of
Biophysics.
5.- TEACHING
ACTIVITIES
a.- Courses (Faculty of
Medicine,
- Biophysics. Lectures (91-95) and Workshops (86-91) for students of the career of Dr. in Medicine and nurses school.
- Quantitative Methods in Biology (1987).
- Cell Biology (Membranes and Radiobiology). Lectures (91-95) and Workshops (86-91) for students of the career of Dr. in Medicine and nurses school.
-Physiology (Excitable Tissues, Cardiophysiology, Pulmonary physiology). Lectures (Excitable Tissues) (91 to 95 - 99 to 2002) and Workshops (86-91) for students of the career of Dr. in Medicine.
-Electrocardiography (2002). Lectures for students of the career of Dr. in Medicine.
b.- Courses (International)
-
-
UNESCO.IBRO.ICRO. “Novel Spectroscopic Approaches to
Neurobiology”. (2000). Faculty Teaching Staff. Lectures. Seminar. Workshop. Ion
channel gating and Calcium channel inactivation. INIBIBB. IBRO. ICRO.
c.-
Laboratory training of science students.
-
2004. Oocyte isolation and ion channel expression. Salkoff’s lab.
-2002:
Patch clamp setups, numerical methods applied to ion channels. Gonzalo Budelli.
Coming next year to
-1999-02:
Patch Clamp, Tissue culture, Basic Molecular Biology. Nicolas Reyes. Currently
at
-1994-95:
Patch clamp, isolation of myocytes. P.Artigas. Currently at
d.- Biophysics Department
Admittance Committee
2001.2002.
Evaluation of tests and qualifications taken by young applicants to join the
Dept. of Biophysics. Faculty of Medicine.
e.- Short Textbooks
-Ionic Channels. (1989). G.Brum and G.Ferreira.
Oficina del libro AEM.
-Excitation-Contraction
Coupling in Skeletal Muscle. (1989).
G.Brum and G.Ferreira. Oficina del libro AEM.
6.- EDUCATIONAL BACKGROUND
A.- M.D. courses (All
courses taken in Faculty of Medicine, University of the Republic,
- Biophysics . (1982).
- Biochemistry . (1982).
- Cytology. (1982).
- Genetics. (1982).
- Histology. (1983).
- Anatomy. (1983).
- Physiology (1984).
- Pathophysiology (1985).
- Semiology (1985).
- Parasitology. (1985).
- Bacteriology and Virology. (1985).
- Pathology. (1986, 87, 93).
- Pharmacology. (1986).
- Medical Clinics (1986-87-93).
- Surgical Clinics. (1986-87-93).
- Histopathology. (86-1987).
- Gynecology and Obstetrics. (1988).
- Psychiatry. (1989).
- Legal Medicine. (1988).
- Preventive Medicine, Epidemiology. (1989).
- Pediatrics. (1994).
B.-
M.Sc. in Biological Sciences courses (All courses taken in Faculty
of Medicine, University of the Republic,
-
Ionic Channels in Biological Membranes. (1988).
-
Electronics in Biology. (1989).
-
Mathematics for biophysicists. (1989).
-
Axonal Transport. (1989). Institute Clemente Estable.
-
Molecular and Cell Biology. (1990-1991).
C.- Extracurricular courses
a.-
In
-English.
(1974-81). Institute Anglo-Uruguayo.
-Biomathematics.
(1983).
-Algebra.
(1985).
-Matemathical
Analysis. (1985).
-Mathematical
Models. (1987).
-Informatics
applied to biology. (1987).
-Statistics
in Medicine. (1987).
-Introduction
to multivariable analysis. (1989).
-Molecular basis of excitability. (1991). PEDECIBA.
-Population Dynamics. (1992). PEDECIBA.
b.- International
-Structural
Basis of muscular contraction. (1988). IVIC
-Transduction
in Biological Systems. (1988). CECS.
-Molecular
and cell biology of ion channels. (1994). Cancún.
-Molecular
approaches to ion channel biology.(1997).
D.- Studies performed to obtain academic positions in Uruguay
To be admitted in academic positions of
degree I and II and join a Department in
E.- Languages
Fluent
reading, writing, listening and speaking abilities – Spanish and English.
Reading,
writing, listening and speaking with some difficulty – Portuguese and French.
7.- GRANTS FUNDED
i.- Research
-Relationship of voltage
and calcium-dependent inactivation of L-type Calcium channels in cardiac cells
and expression systems. FCE.
-Functional Coupling
between L-type Ca channels and BK channels.
ii.- Teaching
-Application
of digital format techniques for teaching Biophysics. (Web design
www.bioltis.fmed.edu.uy, net for students). CSE.
iii.- Organization of Scientific
Events
-International course “Molecular Biology of Ion channels”. PEDECIBA. Uruguay. 2002. G.Ferreira and G.Brum.
-International
Symposium “New Perspectives in Intracellular Calcium and Calcium Sensitive
channels”. Program for Technological Development and CSIC.
8.- FELLOWSHIPS
- Master in Biological Sciences (Biophysics). (1989). (PEDECIBA).
- International course
“Transduction in Biological Systems”. (1988). CECS.
- International course
“Structural basis of muscle contraction”. (1988). IVIC.
-
9.- PROFESSIONAL AFFILIATIONS
- Biophysical Society. Regular
Membership. (1995- )
- Society of Latinamerican
Biophysicists. (1994- )
-
International
- Sociedad Uruguaya de
Biociencias (1998-).
-
Society of General Physiologists. (1996-97).